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| 产品名称： | pG5/T2 |
|---|---|
| 商品货号： | TS201462 |
| Designations： | pG5/T2 |
| GenBank Number： | JO2802 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | A Tzagoloff |
| Applications： | A BglII (-820 nt from translation initiation site) to PstI (-72) fragment has been used to detect a mRNA of about 1.5 kb, presumed to encode mitochondrial fumarase. |
| Vector： | Construct size (kb): 13.69999980926514 |
| Insert： | DNA: genomic Insert lengths(kb): 6.0 Gene product: fumarase(fumarate hydratase class II (fumarase)) FUM1 |
| Insert Size (kb)： | 6.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following sites (separated by approximate distances in kb): HindIII- 0.8- HindIII- 0.1- XhoI- 0.13- BamHI- 0.9- EcoRI- 1.6- HindIII- 1.3- EcoRI. Restriction digests of the clone give the following sizes (kb): SmaI/SalI--7.3, 6.3; SalI--13.7; EcoRV--8.5, 4.4, 0.87; XbaI--8.5, 5.2. A BglII (-820 nt from translation initiation site) to PstI (-72) fragment has been used to detect a mRNA of about 1.5 kb, presumed to encode mitochondrial fumarase. A 2.9 kb HindIII fragment detects 3 mRNAs, with the largest (about 1.9 kb) presumably a product of an unidentified open reading frame 3 to FUM1. The 1.35 kb mRNA is apparently the mRNA for cytoplasmic fumarase. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Wu M, Tzagoloff A. Mitochondrial and cytoplasmic fumarases in Saccharomyces cerevisiae are encoded by a single nuclear gene FUM1. J. Biol. Chem. 262: 12275-12282, 1987. PubMed: 3040736 |