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微信小章| 产品名称： | Trypanosoma lewisi (Kent) Laveran and Mesnil |
|---|---|
| 商品货号： | TS201498 |
| Strain Designations： | New Orleans-67 |
| Application： | Vector borne research |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | rat, Rattus norvegicus, New Orleans, 1967 |
| Product Format： | frozen |
| Type Strain： | no |
| Comments： | Flow cytometric analysis of immune-globulin and complement component C3 on the surface Sensitivity to alternate complement pathway-mediated lysis use of plastic ampoules for freeze preservation |
| Growth Conditions： | Duration: in vivo, laboratory rat Protocol: ATCCNO: 30085 SPEC: Inject the entire thawed contents of the ampule intraperitoneally into an adult rat known to be free of blood parasites. The strain should be monitored, especially after infecting with thawed material. To infect additional rats, draw a drop of tail blood into a 1.0 ml tuberculin syringe containing 0.5 ml of sterile buffered anticoagulant, mix, and inoculate 0.1-0.2 ml of the suspension intraperitoneally/rat. Only a few trypanosomes are needed to infect a non-immune rat. However, do not wait until the blood is swarming with trypanosomes. The time for peak parasitemia may be within 4-5 days if large inocula are given. Peak parasitemia from the thawed material may be slightly sooner or later. |
| Subcultivation： | Protocol: ATCCNO: 30085 SPEC: Inject the entire thawed contents of the ampule intraperitoneally into an adult rat known to be free of blood parasites. The strain should be monitored, especially after infecting with thawed material. To infect additional rats, draw a drop of tail blood into a 1.0 ml tuberculin syringe containing 0.5 ml of sterile buffered anticoagulant, mix, and inoculate 0.1-0.2 ml of the suspension intraperitoneally/rat. Only a few trypanosomes are needed to infect a non-immune rat. However, do not wait until the blood is swarming with trypanosomes. The time for peak parasitemia may be within 4-5 days if large inocula are given. Peak parasitemia from the thawed material may be slightly sooner or later. |
| Name of Depositor： | RG Yaeger |
| Year of Origin： | 1967 |
| References： | Gannon JT, Linke HA. Growth studies on xenic cultures of Entamoeba gingivalis using established media. Int. J. Parasitol. 19: 835-838, 1989. PubMed: 2635159 Simione FP Jr., et al. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502, 1977. PubMed: 891238 Sturtevant JE, Balber AE. Trypanosoma lewisi: restriction of alternative complement pathway C3/C5 convertase activity. Exp. Parasitol. 63: 260-271, 1987. PubMed: 3556206 Sturtevant JE, Balber AE. Flow cytometric analysis of immunoglobulin and complement component C3 on the surface of Trypanosoma lewisi. J. Protozool. 33: 197-203, 1986. PubMed: 3525820 Sturtevant JE, Balber AE. Externally disposed membrane polypeptides of intact and protease- treated Trypanosoma lewisi correlated with sensitivity to alternate complement pathway-mediated lysis. Infect. Immun. 42: 869-875, 1983. PubMed: 6358042 |
| Cross References： | Nucleotide (GenBank) : AJ223566 Trypanosoma lewisi, small subunit ribosomal RNA gene. |