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| 产品名称： | Cercomonas plasmodialis Mylnikov |
|---|---|
| 商品货号： | TS201525 |
| Strain Designations： | AZ-6 |
| Application： | This is the only species of this genus that produces a microplasmodium. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | plant |
| Product Format： | dried |
| Type Strain： | no |
| Comments： | This is the only species of this genus that produces a microplasmodium. The microplasmodium gives rise to large multinucleate cysts. dark brown originally rich matted fibrous plant material, Riverwalk, Tucson, AZ, 1992. |
| Medium： | ATCC® Medium 802: Sonneborns Paramecium medium |
| Growth Conditions： | Temperature: 25.0°C Duration: grown with Enterobacter aerogenes ATCC 13048 and mixed bacteria Protocol: Once the culture is established, the cysts will excyst and the trophozoites will comsume the bacteria in the medium. When the bacterial numbers have been reduced below a certain level, the Cercomonas will once again encyst. The cysts are stabile for at least 1-2 months. Subculture by detaching the cysts from the surface of the flask with a sterile cell scrapper, agitate, and aseptically transfer a 0.1-ml aliquot to a fresh flask containing 10 ml of bacterized medium 802. |
| Subcultivation： | Protocol: Once the culture is established, the cysts will excyst and the trophozoites will comsume the bacteria in the medium. When the bacterial numbers have been reduced below a certain level, the Cercomonas will once again encyst. The cysts are stabile for at least 1-2 months. Subculture by detaching the cysts from the surface of the flask with a sterile cell scrapper, agitate, and aseptically transfer a 0.1-ml aliquot to a fresh flask containing 10 ml of bacterized medium 802. |
| Name of Depositor： | TK Sawyer |
| Chain of Custody： | ATCC < |
| References： | dark brown originally rich matted fibrous plant material, Riverwalk, Tucson, AZ, 1992 |