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| 产品名称： | pBSL142 |
|---|---|
| 商品货号： | TS201605 |
| Designations： | pBSL142 |
| Depositors： | MF Alexeyev |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli DH5 |
| Vector Information： | Size (kb): 4.6690001487731930 DESCRIPTION OF VECTOR COMPONENT: Name of vector: sBSL98 Intact vector size: 3.100 Type of vector: phagemid Vector end: mLuI Vector end: mLuI Cloning sites: Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI NotI/EagI BstXI SacII SacI mLuI Construction: pBluescript Host range: Escherichia coli Features (with orientation and position when available): MCS: KpnI...SacI mLuI, -> MCS: mLuI KpnI...SacI, -> replicon: pMB1 marker(s): ampR, replicon: f1, Cross references: Vector: pBSL142 (phagemid) Promoters: Promoter for in vitro transcription f1T7 (phi10) Construction: pBluescript Marker(s):ampR,gtmR Construct size (kb): 4.669000148773193 Features: marker(s): ampR promoter for in vitro transcription: f1T7 (phi10) replicon: f1 replicon: pMB1 MCS: KpnI...SacI MluI MCS: MluI KpnI...SacI coding sequence: aacC1 (gtmR) |
| Applications： | contains easily purifiable cassette(s) for construction omega - aacC1 |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.8, 1.6; EcoRI--2.95, 1.75; KpnI--2.8, 1.65; MluI--2.9, 1.6; SstI--2.8, 1.7. Plasmid useful for generation of marked deletions and insertions in cloned DNA in vitro. Contains an omega - Gm element (aacC1, gtmR) flanked by multiple cloning sites duplicated in tandem orientation. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |