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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS201772 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | SG927 |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Preceptrol®： | no |
| Genotype： | F- londelta100 tsx::Tn10 malPQ::Tn5 lacU169 galK2 thr-1 leu-6 his-4 argE3 ilu(Ts) recA441 prsL31 sulA11 htpR(Am) lambda- |
| Type Strain： | no |
| Comments： | Resistant to T6 infection. One of a series of host strains (ATCC 39623, 39624, 39627, 39628) defective in proteolysis for use in large-scale production of genetically engineered proteins with heat-inducible expression control sequences. Resistant to T6 infection. A P1 lysate was made from a strain carrying tsx::Tn10 and lon delta100. This lysate was used to transduce these mutations into GW4701. |
| Medium： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 30.0°C |
| Name of Depositor： | Harvard Medical School, LP Casson, Harvard Medical School |
| U.S. Patent Number： | |
| References： | Goldberg AL, et al. Hosts and methods for producing recombinant products in high yields. US Patent 4,758,512 dated Jul 19 1988 |