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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS201807 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | KS272 |
| Application： | Transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | F- delta(lacIPOZY)X74 galE galK thi rpsL deltaphoA |
| Type Strain： | no |
| Comments： | Host strain (ara+) for use with the pBAD series of expression vectors (ATCC 87393 - 87402). Metabolism of arabinose results in very rapid repression of expression for pBAD vectors. Induction of expression in pBAD vectors can be maintained at high levels. Induction response is not linear with respect to inducer (arabinose) concentrations. |
| Medium： | ATCC® Medium 1445: Nutrient agar or broth with 20 mcg/ml streptomycin |
| Growth Conditions： | Temperature: 37°C
Atmosphere: Aerobic |
| Name of Depositor： | L Guzman, J Beckwith |
| Special Collection： | NCRR Contract |
| References： | Strauch KL, Beckwith J. An Escherichia coli mutation preventing degradation of abnormal periplasmic proteins. Proc. Natl. Acad. Sci. USA 85: 1576-1580, 1988. PubMed: 3278319 Guzman LM, et al. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177: 4121-4130, 1995. PubMed: 7608087 |