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| 产品名称： | Schizosaccharomyces pombe Lindner |
|---|---|
| 商品货号： | TS201851 |
| Deposited As： | Schizosaccharomyces pombe Lindner, teleomorph |
| Strain Designations： | FY191 |
| Application： | produces protein tetracycline repressor transformation host host required for regulated expression of sequences cloned into vectors containing a tet operator and a CaMV promoter (pSLF101, ATCC 87619; pSLF102, ATCC 87620). The CaMV-tet vector in the strain FY191 is repressed in the absence of tetracycline and induced by addition of tetracycline to 10 ug/ml. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Genotype： | h- ura4-D18 leu1-32 ade6-704::pSLF104 sup3-5 adh-tetR |
| Preceptrol®： | no |
| Mating Type： | h- |
| Ploidy： | haploid |
| Comments： | The vector contains the marker sup3-5, which suppresses ade6-704, and the tet repressor gene under control of the S. pombe adh promoter. produces the tetracycline repressor constitutively |
| Medium： | ATCC® Medium 2064: YES MEDIUM |
| Growth Conditions： | Temperature: 25.0°C |
| Name of Depositor： | SL Forsburg |
| Special Collection： | NCRR Contract |
| Isolation： | generated by integrating the vector pSLF104 (ATCC 87621) into a strain of genotype h- ade6-704 leu1-32 ura4-D18 |
| References： | Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516 |