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| 产品名称： | pKC203 |
|---|---|
| 商品货号： | TS201898 |
| Designations： | pKC203 |
| Depositors： | Eli Lilly & Co., MD Jones, Eli Lilly & Co. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli JR225 Distribution host: Escherichia coli JR225 |
| Vector Information： | Size (kb): 15.0000000000000000 Vector: pKC203 (plasmid) Marker(s):G418R,hygR Construct size (kb): 15.0 Features: marker(s): hygR, G418R replicon: pMB1 |
| Applications： | contains easily purifiable cassette(s) for construction shuttle vector |
| Comments： | The 2.5 kb SalI/BglII fragment contains internal sites for AvaI, PvuI, EcoRI, XhoI, BstEII (one site each) and PstI, SacI (2 sites each). Restriction digests of the clone give the following sizes (kb): EcoRI--23, 16; BglII--17, 12, 6.0, 4.7, 3.9; SalI--18, 15, 11. This pattern differs from the information supplied by the depositor. The hygromycin B and G418 resistance structural genes and control elements can be isolated on the 7.5 kb BglII fragment or the 2.5 kb SalI/BglII fragment. The 1.5 kb BglII/SacI fragment contains hygR. The 1.65 EcoRI/SalI fragment contains G418R. The 7.5 kb BglII fragment contains a prokaryotic replicon and can be self-ligated to form plasmid pSC701. This is a vector with easily isolated hygromycin B and G418 resistance structural genes and control elements for use in constructing other plasmids. |
| Media： | ATCC® Medium 1952: LB medium (ATCC medium 1065) with 200 mcg/ml hygromycin B |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Ingolia TD. DNA for directing transcription and expression of structural genes. US Patent 4,559,302 dated Dec 17 1985 Santerre RF, Rao RN. Recombinant DNA cloning vectors and the eukaryotic and prokaryotic transformants thereof. US Patent 4,727,028 dated Feb 23 1988 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |