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| 产品名称： | Pst/Sma2 |
|---|---|
| 商品货号： | TS202140 |
| Designations： | Pst/Sma2 |
| GenBank Number： | D00657 |
| Species： | Red clover mottle virus |
| Depositors： | M Shanks |
| Vector： | Construct size (kb): 10.10000038146973 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.900000095367432 Gene product: 24K protease |
| Insert Size (kb)： | 2.900 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--7.4, 1.7, 1.0; PvuII--6.8, 1.6, 0.9, 0.7; PstI--10.5; PstI/EcoRI--8.5, 1.6; PstI/SstI--7.2, 1.7, 1.2; TaqI--2.0, 1.4, 1.05, 0.95, 0.8, 0.7, 0.6 + smaller. The insert contains the following restriction sites (approximate kb from the 5 end): PvuII--1.3, 2.1; TaqI--1.4, 2.8; BamHI--1.7, 2.2. The 5 end of the insert corresponds to the PstI site approximately 1.6 kb from the 5 end of the RNA. |
| Classification： | Comovirus |
| References： | Shanks M, Lomonossoff GP. The primary structure of the 24K protease from red clover mottle virus: implications for the mode of action of comovirus proteases. J. Gen. Virol. 71: 735-738, 1990. PubMed: 2179468 |