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| 产品名称： | PNN414 pYACneo |
|---|---|
| 商品货号： | TS202152 |
| Designations： | PNN414 pYACneo |
| Depositors： | RW Davis |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 15.6999998092651400 Vector: PNN414 (YAC) Promoters: Promoter SUP4 Construction: pYAC4, neo of pSV2neo Marker(s):TRP1,URA3,ampR,neoR,HIS3,SUP4 Construct size (kb): 15.69999980926514 Features: marker(s): ampR, neoR, TRP1, URA3, HIS3, SUP4 promoter: SUP4 replicon: pMB1, ARS1 centromere: CEN4 |
| Applications： | YL-type (YAC) shuttle vector integrating vector shuttle vector vector for constructing genomic libraries |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--15.9; XhoI--11.0, 3.4, 1.0; BamHI--14.0, 1.85; BamHI/EcoRI--8.2, 5.8, 1.85; SalI/BamHI--7.4, 6.6, 1.85. To facilitate chromosome walking, the ends of the insert can be recovered in E. coli. The artificial chromosomal DNA can be digested with XhoI, ligated, and used to transform E. coli to amp or kan resistance. Cloning into the EcoRI site insertionally inactivates SUP4. G418 resistance is expressed in mammalian cell lines to permit genetic complementation experiments. Constructed by Christopher Traver. XhoI linkers were ligated to the blunt ended 4.2 kb PvuI/BamHI fragment of pSV2neo (with neomycin resistance and ColE1 origin). This was then cloned into the SalI site of pYAC4. Contains telomer elements from Tetrahymena. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Traver CN, et al. Rapid screening of a human genomic library in yeast artificial chromosomes for single-copy sequences. Proc. Natl. Acad. Sci. USA 86: 5898-5902, 1989. PubMed: 2668948 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |