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| 产品名称： | pJAJ7 |
|---|---|
| 商品货号： | TS202180 |
| Designations： | pJAJ7 |
| Depositors： | JT Beatty |
| Biosafety Level： | 1 |
| Host： | E. coli JM83 |
| Vector Information： | Size (kb): 11.1999998092651400 Vector: pJAJ7 (plasmid) Promoters: Promoter puf (rxcA) Construction: pJAJ103 (from pRK404), puf promoter Marker(s):tetR Construct size (kb): 11.19999980926514 Features: marker(s): tetR promoter: puf (rxcA) replicon: RK2 |
| Applications： | expression vector shuttle vector vector permitting construction of fusion proteins |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--13.0; SstI--13.0; BamHI--13.0. Expression can be modulated as oxygen starvation of R. capsulatus stimulates transcription from the puf promoter. Can be mobilized by conjugation from R. capsulatus into Escherichia coli and other species that make up the host range of RK2. A broad host range vector for the expression of heterologous genes in Rhodobacter capsulatus under the control of the R. capsulatus puf promoter. pJAJ7 (TS202180) and pJAJ9 (ATCC 37649) differ only in the orientation of the puf promoter. The XhoI/BamHI fragment of pJAJ21 containing the puf promoter and the 5 end of the B870beta gene of Rhodobacter capsulatus was cloned into the BamHI site of the broad host range vector pJAJ103. |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Johnson JA, et al. Expression of cellulase genes in Rhodobacter capsulatus by use of plasmid expression vectors. J. Bacteriol. 167: 604-610, 1986. PubMed: 3090019 |
| Shipped： | frozen |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the plasmid |