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微信小章| 产品名称： | pUCSV40-CPC/MEN-1 molecular clone of Simian virus 40, SV-40 |
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| 商品货号： | TS202274 |
| Designations： | pUCSV40-CPC/MEN-1 molecular clone of Simian virus 40, SV-40 |
| GenBank Number： | AF156108 |
| Species： | Simian virus 40, Simian virus 40 |
| Depositors： | JA Lednicky, JS Butel |
| Isolation： | Contains a full-length genome of SV-40 strain CPC/MEN cloned into the EcoRI site of pUC-19. SV40-CPC/MEN was isolated and cloned from virus stock derived from choroid plexus carcinoma DNA sample 12 (from a 4 yr old child). |
| Applications： | contains sequence |
| Vector： | Construct size (kb): 7.86
DESCRIPTION OF VECTOR COMPONENT xa0xa0xa0xa0xa0xa0xa0 Type of vector:xa0 E. coli xa0xa0xa0xa0xa0xa0xa0 Name of vector: pUC-19 xa0xa0xa0xa0xa0xa0xa0 Intact vector size (kb):xa0 2.68 kb |
| Insert： | DNA: cDNA Insert lengths(kb): 5.179999828338623 Tissue: choroid plexus carcinoma Gene product: SV40-CPC/MEN-1 |
| Insert Size (kb)： | 5.180 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | It has an archetypal viral regulatory region and was tested by lipofection of EcoRI-linearized plasmid DNA into TC7 cells, with cells showing subsequent production of typical SV-40 CPE. Lipofection into TC7 and Butel lab CV-1 cells was verified. Contains a full-length genome of SV-40 strain CPC/MEN cloned into the EcoRI site of pUC-19. SV40-CPC/MEN was isolated and cloned from virus stock derived from choroid plexus carcinoma DNA sample 12 (from a 4 yr old child). |
| Classification： | Polyomaviridae, Polyomavirus |
| References： | Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955 Lednicky JA, et al. Natural simian virus 40 strains are present in human choroid plexus and ependymoma tumors. Virology 212: 710-717, 1995. PubMed: 7571441 |