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| 产品名称： | pBT-1 |
|---|---|
| 商品货号： | TS202326 |
| Designations： | pBT-1 |
| Species： | human immunodeficiency virus - 1 |
| Depositors： | SM Watanabe |
| Vector： | Construct size (kb): 11.77999973297119 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC18 Intact vector size: 2.686 Type of vector: plasmid Vector end: SstI Vector end: SstI Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: HindIII...EcoRI, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: L08752 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human immunodeficiency virus - 1 Gene name: genome, full-length Contains complete coding sequence?: U Strain: BRU Type of DNA: cDNA Insert end: SstI Insert end: SstI Insert size (kb): 9.09 Cross references: Insert lengths(kb): 9.09000015258789 |
| Insert Size (kb)： | 9.090 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen |
| Comments： | Restriction digests of the clone give the following sizes (kb): SstI--9.0, 2.7; EcoRI--6.2, 4.0, 1.0; HindIII--6.2, 3.1, 1.4, 0.64. Contains the proviral genome from bp 33 to 9129 (numbered according to Wain-Hobson et al., 1985 (Cell 40:9-17). The insert is oriented with the 5 end closer to the EcoRI site in the polylinker of the vector. Cloned from human immunodeficiency virus 1BRU. Contains the proviral genome from bp 33 to 9129. Cloned from human immunodeficiency virus 1BRU. Contains the proviral genome from bp 33 to 9129. This is a subclone of lambdaJ19. |
| References： | Watancke SM. Expression and diagnostic use of pENV-3 encoded peptides which are immunologically reactive with antibodies to LAV. US Patent 4,784,941 dated Nov 15 1988 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |