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| 产品名称： | pCDK4 |
|---|---|
| 商品货号： | TS202337 |
| Designations： | pCDK4 |
| GenBank Number： | L11007 |
| Species： | Rattus norvegicus, rat |
| Depositors： | T Weaver |
| Vector： | Construct size (kb): 5.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.399999976158142 Tissue: fetal lung (21 day old) Gene product: cyclin-dependent kinase 4( p34/cdk4, cell division kinase 4) CDK4 |
| Insert Size (kb)： | 1.400 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains the following restriction sites (approximate kb from the 5 end): PvuII--0.01, 0.25, 0.78; KpnI--0.65; PstI--0.68, 0.99. Restriction digests of the clone give the following sizes (kb): HindIII--5.5; PstI--4.4, 0.8, 0.3; NotI--5.5; XbaI/HindIII--4.1, 1.4; XbaI--4.1, 1.4. Does not express the protein when transfected into COS cells. The orientation 3 - 5 relative to the promoter. |
| References： | Cho FS, et al. Cloning of the rat cyclin-dependent kinase 4 cDNA: implication in proliferation-dependent expression in rat tissues. Biochem. Biophys. Res. Commun. 191: 860-865, 1993. PubMed: 8466525 Tim Weaver, personal communication |