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| 产品名称： | Y-227 |
|---|---|
| 商品货号： | TS202388 |
| Designations： | Y-227 |
| Species： | Homo sapiens, human |
| Depositors： | U Muller |
| Vector： | Construct size (kb): 7.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 2.799999952316284 Tissue: 49,XXXXY cell line Gene product: DNA Segment, single copy DYS185 |
| Insert Size (kb)： | 2.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.2; AvaI--7.2; PstI--7.2; EcoRI--7.2; HindIII--4.4, 2.8. Y-227 hybridizes to a BssHII fragment of 2.7 Mb also detected by Y-280. Y-227 hybridizes to a NotI fragment of 3 Mb which is not detected by Y-280. In HindIII digests, Y-227 hybridizes to a 2.8 kb Y-specific fragment and a smaller non-Y fragment. Each is single copy. The Y-specific band was detected in 8 of 11 46,XX males, but not in 2 46,XX hermaphrodites or 1 46,XY gonadal dysgenesis patient. Isolated from a flow-sorted human Y chromosome library enriched for DNA from the Y euchromatic portion. |
| References： | Muller U, et al. Deletion mapping of the testis determining locus with DNA probes in 46,XX males and in 46,XY and 46,X,dic(Y) females. Nucleic Acids Res. 14: 6489-6505, 1986. PubMed: 3748818 Gaunt SJ. Mapping of testis-determining locus on Yp by the molecular genetic analysis of XX males and XY females. Development 101: 51-58, 1987. PubMed: 88211484 Muller U, Lalande M. A physical map of the human Y-chromosome short arm. Genomics 7: 517-523, 1990. PubMed: 1974877 |