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| 产品名称： | pCAT/LIC |
|---|---|
| 商品货号： | TS202396 |
| Designations： | pCAT/LIC |
| Depositors： | RS Haun |
| Other IDs： | Nucleotide (GenBank) : U25266 Ligation-independent promoter-cloning vector pLUC/LIC, complete sequence. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.4000000953674320 Vector: pCAT/LIC (plasmid) Construction: pCAT-Basic Marker(s):ampR Construct size (kb): 4.400000095367432 Features: marker(s): ampR replicon: pMB1 reporter group: cat coding sequence MCS: HindIII...XbaI terminator: SV40 polyadenylation |
| Applications： | promoter-cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): ApaI--4.4; HindIII--4.4; XbaI--4.4. Preparation of the vector for cloning includes linearization with ApaI, gel purification of the linearized vector, and treatment with T4 DNA polymerase in the presence of dATP. The target sequence can be amplified using sequence specific primers modified at the 5 end to contain an additional 13 nt complementary to the vector. The forward primer should contain 5-CCTGCTCGTCTGA-3 followed by 12-15 nt target-specific sequence. The reverse primer should contain 5-GGTGGTGCTCTGA-3 followed by 12-15 nt target-specific sequence. Sequences amplified using these primers are also compatible with the pLUC/LIC vector (ATCC 87045). Ligation-independent promoter-cloning vector, using chloramphenicol acetyltransferase as the reporter group. The order of the major features in the plasmid is: ampR - pMB1 ori - HindIII - ApaI - XbaI - cat coding sequence - SV40 splice site and polyadenylation. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Haun RS, et al. Characterization of the human ADP-ribosylation factor 3 promoter: transcriptional regulation of a TATA-less promoter. J. Biol. Chem. 268: 8793-8800, 1993. PubMed: 8473323 Haun RS, et al. Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors. BioTechniques 13: 515-518, 1992. PubMed: 1362067 |
| Shipped： | freeze-dried |