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| 产品名称： | pERT87-30 |
|---|---|
| 商品货号： | TS202488 |
| Designations： | pERT87-30 |
| Species： | Homo sapiens, human |
| Depositors： | LM Kunkel |
| Vector： | Construct size (kb): 4.599999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 1.799999952316284 Gene product: DNA Segment, single copy (and related walk clones (within DMD gene)) DXS164 Alleles: N1, N2, A1, A2, B1, D1, D2, E2, G1, J1, J2, K1, O1, O2, E1, G2, K2, B2 |
| Insert Size (kb)： | 1.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--2.7, 1.8; EcoRI--4.6; BamHI--4.6; PstI--4.6; SmaI--4.6; consistent with deposited DNA. This construct is not stable when freeze-dried and is distributed from liquid nitrogen stock. This clone is from within the DMD locus. This clone is 54.5 kb more pter than pERT87-15. The polymorphisms detected by this probe are contained within the DMD locus. The DNA must be extensively nicked to get efficient transfer of the large fragment. |
| References： | Koenig M, et al. Complete cloning of the Duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals. Cell 50: 509-517, 1987. PubMed: 3607877 Monaco AP, et al. Localization and cloning of Xp21 deletion breakpoints involved in muscular dystrophy. Hum. Genet. 75: 221-227, 1987. PubMed: 2881877 Louis M Kunkel, personal communication |