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| 产品名称： | pAAV/Ad pAd/AAV |
|---|---|
| 商品货号： | TS202548 |
| Designations： | pAAV/Ad pAd/AAV |
| GenBank Number： | J01901 L43079 |
| Species： | Adenovirus |
| Depositors： | Trustees of Princeton University, TE Shenk, Trustees of Princeton University |
| Applications： | in another host, produces protein capsid protein in another host, produces protein replication protein integration helper plasmid |
| Vector： | Construct size (kb): 7.800000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 4.300000190734863 Gene product: capsid protein cap Target Gene: , capsid protein, replication protein |
| Insert Size (kb)： | 4.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): ClaI & EcoRI--3.4, 2.6, 1.8; HindIII--6, 1.8; XbaI--4.2, 3.6. AAV2 insert contains the following restriction sites (approximate kb from the 5 XbaI site): BamHI--0.85; BglI--0.35, 4.2; EcoRI--1.6, 1.8; HindIII--1.7; KpnI--1.7, 4.0; PstI--0.3, 1.75, 4.05; SacI--0.6; SalI--1.25; XhoI--2.05, 2.2. Helper virus for packaging helper-free recombinant AAV. Plasmid provides all trans functions (replication and capsid proteins) necessary for packaging AAV. Useful for creating recombinant AAV in conjunction with the cloning vector pAAV/neo (ATCC 68065). Constructed by replacing the inverted terminal repeats of the AAV2 genome with inverted repeats from adenovirus type 5. The poison sequence of pBR322 was also removed. The AAV portion of the insert can be excised by digestion with XbaI. |
| References： | Shenk TE, et al. Helper-free stocks of recombinant adeno-associated virus vectors. US Patent 5,436,146 dated Jul 25 1995 Samulski RJ, et al. Helper-free stocks of recombinant adeno-associated viruses: normal integration does not require viral gene expression. J. Virol. 63: 3822-3828, 1989. PubMed: 2547998 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |