| 产品名称: | pmigf1-2 pPE1 |
|---|---|
| 商品货号: | TS202588 |
| Designations: | pmigf1-2 pPE1 |
| GenBank Number: | X04480 |
| Species: | Mus musculus, mouse |
| Depositors: | GI Bell |
| Vector: | Construct size (kb): 4.0 |
| Insert: | DNA: cDNA Insert lengths(kb): 0.7200000286102295 Tissue: liver (8-week-old) Gene product: insulin-like growth factor 1 Igf1 Alleles: b, s |
| Insert Size (kb): | 0.720 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | The insert includes these restriction sites (nucleotide position according to the published sequence): BamHI--585. Restriction digests of the clone give the following sizes (kb): EcoRI--3.6, 0.8; HindIII--4.3; BamHI--3.7,0.6, PstI--4.3, SacI--4.3. The insert contains the complete coding sequence for mouse preproIGF-IA, including 5 and 3 untranslated sequences. preproIGF-IA is one of two processing products of a common primary transcript. The insert detects transcripts of about 0.8 (major), 1.5, and 7.0 kb. |
| Classification: | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References: | Bell GI, et al. Sequences of liver cDNAs encoding two different mouse insulin-like growth factor I precursors. Nucleic Acids Res. 14: 7873-7882, 1986. PubMed: 3774549 Justice MJ, et al. A genetic linkage map of mouse chromosome 10: localization of eighteen molecular markers using a single interspecific backcross. Genetics 125: 855-866, 1990. PubMed: 1975791 |