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| 产品名称： | pENV9 |
|---|---|
| 商品货号： | TS202720 |
| Designations： | pENV9 |
| GenBank Number： | M15654 |
| Species： | human immunodeficiency virus - 1 |
| Depositors： | E.I. du Pont, L Ivanoff, E.I. du Pont |
| Applications： | produces protein env |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: genomic Insert lengths(kb): 0.8579999804496765 Gene product: env env Target Gene: env |
| Insert Size (kb)： | 0.858 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/BglII--5.0, 0.9; BglII/HindIII--5.3, 0.6; BamHI/EcoRI--4.3, 2.1. The insert contains a HindIII site 0.52 kb downstream of the 5 end BglII site. Transcription from the trp promoter produces a fusion protein including 54 aa from the 5 poliovirus 3C protease (encoded by the vector), 46 aa from the C-terminal portion of gp120, and 240 aa from the N-terminal portion of gp41. The recombinant protein is recognized by monoclonal antibodies specific to gp41 and gp120. The vector is a pBR322 derivative with trp operator/promoter, Shine-Dalgarno and protease 3C sequences inserted at the ClaI site. |
| References： | Ivanoff LA, Petteway SR. Human immunodeficiency virus antigen. US Patent 4,861,707 dated Aug 29 1989 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |