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| 产品名称： | pAc 6C2B23 DH5OC |
|---|---|
| 商品货号： | TS202772 |
| Designations： | pAc 6C2B23 DH5OC |
| GenBank Number： | M38245 |
| Species： | Canine parvovirus |
| Depositors： | Cornell Univ. |
| Applications： | This plasmid was used to construct CPV C62B23, ATCC VR-2209, which produces immunogenic VP-2 when inoculated into Spodoptera frugiperda cells. The BamHI linker site was used to insert the canine parvovirus VP-2 gene from strain C-780916 (CPV-916), ATCC VR-953, so that transcription of the VP-2 gene was under the control of the polyhedrin gene promoter. |
| Vector： | Construct size (kb): 11.5 |
| Insert： | DNA: genomic Insert lengths(kb): 2.170000076293945 Gene product: capsid protein VP-2 |
| Insert Size (kb)： | 2.170 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: Frozen |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--6.7, 3.7, 1.1; BamHI--9.1, 2.2; PvuII--3.7, 3.6, 2.2, 1.6, 0.6; EcoRI/EcoRV--3.7, 3.7, 2.5, 1.4. The insert is from nt 2750 to nt 4990 of the GenBank sequence, but it differs from the GenBank sequence at the following nt (GenBank to pAc6C2B23 change): 3065 (from C to A), 3094 (from T to A), 3480 (from A to G), 3855 (from C to T), 3596 (from T to C), 3657 (from C to T), 3753 (from A to G). This plasmid was used to construct CPV C62B23, ATCC VR-2209, which produces immunogenic VP-2 when inoculated into Spodoptera frugiperda cells. A 7.0 kb EcoRI fragment from AcNPV 1A strain, containing the polyhedrin gene, was inserted into the EcoRI site. Nucleotides -7 to +461 were deleted (where +1 is the A of the polyhedrin ATG), and a 23 base BamHI linker was inserted. This vector provides the strong polyhedrin promoter, polyhedrin terminator signals, and sufficient flanking sequence to promote homologous recombination in cells co-transfected with baculovirus. Constructed from a pUC19 derivative from which the BamHI recognition site had been removed. The BamHI linker site was used to insert the canine parvovirus VP-2 gene from strain C-780916 (CPV-916), ATCC VR-953, so that transcription of the VP-2 gene was under the control of the polyhedrin gene promoter. |
| Classification： | Parvoviridae, Parvovirus, Feline panleukopenia virus |
| References： | Wood HA, Parrish CR. Subunit canine parvovirus vaccine. US Patent 4,971,793 dated Nov 20 1990 Luckow VA, Summers MD. Trends in the development of baculovirus expression vectors. Bio-Technology 6: 47-55, 1988. Smith GE, et al. Production of human beta interferon in insect cells transfected with a baculovirus expression vector. Mol. Cell. Biol. 3: 2156-2165, 1983. PubMed: 6318086 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |