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| 产品名称： | YEplac195 |
|---|---|
| 商品货号： | TS202830 |
| Designations： | YEplac195 |
| Depositors： | RD Gietz |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.241 Vector: YEplac195 (plasmid) Promoters: Promoter lac Construction: pUC19 Marker(s):URA3,ampR Construct size (kb): 5.241 Features: insert detection: lacZ marker(s): URA3 marker(s): ampR promoter: lac replicon: 2 micron ori replicon: pMB1 MCS: HindIII...EcoRI |
| Applications： | YE-type (episomal) shuttle vector vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase |
| Comments： | This is a YE-type (yeast episomal) vector with the URA3 marker. The PstI restriction site from the URA3 gene of S. cerevisiae were removed by using oligo-directed mutagenesis. One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1). Restriction digests of the clone give the following sizes (kb): AvaI--4.2, 0.7; EcoRV--5.0; PstI--5.0. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106 |
| Related Products： | component of:ATCC 87623 component of:ATCC 87638 |
| Shipped： | frozen |
| Shipping Information： | xa0Freeze dried E. coli containing the plasmid |