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| 产品名称： | pT7/Nco |
|---|---|
| 商品货号： | TS202853 |
| Designations： | pT7/Nco |
| Depositors： | RS Haun |
| Other IDs： | Nucleotide (GenBank) : U25269 Expression cloning vector pT7/Nde, complete sequence. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.9000000953674320 Vector: pT7/Nco (plasmid) Promoters: Promoter SP6 Construction: pGEM-3, pET-3d Marker(s):ampR Construct size (kb): 2.900000095367432 Features: marker(s): ampR promoter: SP6 promoter: T7 (phi10) replicon: pMB1 MCS: HindIII...XbaI ribosome-binding site: gene 10 |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--2.9; BamHI--2.9; NcoI--2.9. The NcoI site (CCATGG) includes the initiator methionine codon. The NcoI cloning site can also be used in conjunction with the downstream BamHI site. Differs from pT7/NdeI (ATCC 87050) only in the cloning site and reading frame for the insert. Expression vector, maintained at high copy number. The order of the major features of the plasmid is: SP6 - gene 10 RBS - T7 - NcoI - BamHI - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Hong JX, et al. Effect of ADP-ribosylation factor amino-terminal deletions on its GTP-dependent stimulation of cholera toxin activity. J. Biol. Chem. 269: 9743-9745, 1994. PubMed: 8144566 |
| Shipped： | freeze-dried |