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| 产品名称： | pJM6 phagemid in E. coli |
|---|---|
| 商品货号： | TS202965 |
| Designations： | pJM6 phagemid in E. coli |
| Depositors： | TW Jeffries |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.744 Vector: pJM6 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pJM4, pBluescriptIIKS+ Marker(s):URA3,ampR Construct size (kb): 5.74399995803833 Features: marker(s): URA3 marker(s): ampR promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: ARS2 replicon: f1 replicon: pMB1 MCS: SacI/.../EcoRI |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--2.9 doublet; PstI/XhoI--2.9 doublet; XbaI/XhoI--2.9 doublet; HindIII--6.0; XhoI--6.0. The insert contains the following restriction sites (approximate kb from the 5 end): HpaII--0.76, 1.08; BsmI--0.97; HindIII--1.77; AgeI--1.94. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Yang VW, et al. High-efficiency transformation of Pichia stipitis based on its URA3 gene and a homologous autonomous replication sequence, ARS2. Appl. Environ. Microbiol. 60: 4245-4254, 1994. PubMed: 7811063 |
| Shipped： | freeze-dried |