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| 产品名称： | YRp7-17camp |
|---|---|
| 商品货号： | TS203033 |
| Designations： | YRp7-17camp |
| Depositors： | H Feldmann |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.8200001716613770 Vector: YRp7-17camp (plasmid) Promoters: Promoter cml Construction: YRp7, pBR325 Marker(s):TRP1,ampR Construct size (kb): 6.820000171661377 Features: marker(s): ampR, TRP1 promoter: cml replicon: pMB1, ARS1 terminator: CAT |
| Applications： | YR-type (replicating) shuttle vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--7.0; EcoRI--4.6, 1.4, 0.82; PvuII--4.9, 2.05. The depositor recommends using YRp7-17camp (TS203033) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al. One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast. A 5.47 kb ClaI/BamHI fragment of YRp7 was ligated to a 1.35 kb Sau96I fragment of pBR325. Contains the complete bacterial cml gene. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257 |
| Shipped： | freeze-dried |