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微信小章| 产品名称： | pPB102 |
|---|---|
| 商品货号： | TS203047 |
| Designations： | pPB102 |
| Depositors： | HM Temin |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.3299999237060550 Vector: pPB102 (plasmid) Promoters: Promoter SNV LTR Construction: pPB101 (ATCC 45012) Marker(s):ampR Construct size (kb): 8.329999923706055 Features: marker(s): ampR promoter: SNV LTR replicon: pMB1 |
| Applications： | expression vector shuttle vector |
| Comments： | A plasmid shuttle vector constructed from pPB101 (ATCC 45012). Cloned chicken virus (SNV-60B Sal). Constructed by inserting: BglII deletion of complete SNV (HindIII linkers replace BglII site) in A plasmid shuttle vector constructed from pPB101 (ATCC 45012) with BglII deletion and HindIII linkers replacing repaired BlgII sites. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Watanabe S, Temin HM. Construction of a helper cell line for avian reticuloendotheliosis virus cloning vectors. Mol. Cell. Biol. 3: 2241-2249, 1983. PubMed: 6318091 Chen IS, Temin HM. Substitution of 5 helper virus sequences into non-rel portion of reticuloendotheliosis virus strain T suppresses transformation of chicken spleen cells. Cell 31: 111-120, 1982. PubMed: 6297744 Bandyopadhyay PK, Temin HM. Expression of complete chicken thymidine kinase gene inserted in a retrovirus vector. Mol. Cell. Biol. 4: 749-754, 1984. PubMed: 6325895 ORear JJ, et al. Infectious and noninfectious recombinant clones of the provirus of SNV differ in cellular DNA and are apparently the same in viral DNA. Cell 20: 423-430, 1980. PubMed: 6248244 |
| Shipped： | freeze-dried |