微信小陈
微信小章| 产品名称： | BCHE |
|---|---|
| 商品货号： | TS203066 |
| Designations： | BCHE |
| GenBank Number： | J02964 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.299999952316284 Tissue: basal ganglia Gene product: butyrylcholinesterase(cholinesterase (serum) 1) BCHE |
| Insert Size (kb)： | 2.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI/SstI--2.8, 2.3; EcoRI--4.7, 0.3; XbaI--5.0; HincII--5.0; BglII--3.3, 1.9. The insert contains the following restriction sites (kb from the 5 end): BglII--0.1, 1.9; EcoRI--0.3; XbaI--0.9; StuI--1.35; HincII--1.7; SspI--1.8, 2.0, 2.3. and BglII sites have been introduced 7 nt upstream of the codon for Met -28, and 6 nt downstream of the TAA stop codon. The complete coding sequence can thus be excised as a 1.8 kb BglII fragment. |
| References： | Arpagaus M, et al. Structure of the gene for human butyrylcholinesterase. Evidence for a single copy. Biochemistry 29: 124-131, 1990. PubMed: 2322535 Allderdice PW, et al. The cloned butyrylcholinesterase (BCHE) gene maps to a single chromosome site, 3q26. Genomics 11: 452-454, 1991. PubMed: 1769657 McTiernan C, et al. Brain cDNA clone for human cholinesterase. Proc. Natl. Acad. Sci. USA 84: 6682-6686, 1987. PubMed: 3477799 Oksana Lockridge, personal communication |