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| 产品名称： | pJAK17 JK397 |
|---|---|
| 商品货号： | TS203128 |
| Designations： | pJAK17 JK397 |
| Depositors： | JA Kornacki |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 12.6000003814697300 Vector: pJAK17 (plasmid) Promoters: Promoter tac Construction: pMMB67HE (ATCC 37623), pSa Marker(s):cmlR Construct size (kb): 12.60000038146973 Features: marker(s): cmlR promoter: tac replicon: RSF1010 replicon: oriT replicon: oriV repressor gene: lacIq MCS: HindIII...EcoRI transcription terminator: rrnB |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--12.6; HindIII--12.6; SphI--9.0, 3.6. A shuttle vector permitting autoregulated high-level expression in all gram-negative bacteria. Compatible with common E. coli cloning vectors, as well as vectors derived from IncP and IncW plasmids. Can be mobilized by conjugative IncP helper plasmids e.g. pRK2013 (ATCC 37159), pUB307 into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria. Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation. Differs from pJAK16 (ATCC 77291) only in the orientation of the polylinker. |
| Media： | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Furste JP, et al. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48: 119-131, 1986. PubMed: 3549457 Jon A Kornacki, personal communication |
| Related Products： | component of:ATCC 87055 |
| Shipped： | frozen |
| Shipping Information： | Freeze dried plasmid in E. coli |