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| 产品名称： | pF5 |
|---|---|
| 商品货号： | TS203193 |
| Designations： | pF5 |
| Species： | Homo sapiens, human |
| Depositors： | KM Gray, GF Saunders |
| Vector： | Construct size (kb): 4.599999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 0.6819999814033508 Tissue: biopsy Gene product: alpha satellite, XbaI D*Z2 |
| Insert Size (kb)： | 0.682 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | XbaI (insert) and HindIII(vector) ends filled in and blunt-end ligated so insert can be removed by XbaI digest. Strongest hybridization to fragments of 680, 1020, 850, and 1870 bp generated by XbaI-cut DNA. Sequence of pE1 differs from pF5 by 15 nucleotides. Hybridizes with DNA from chimpanzee, gorilla, weakly with orangutan, but not with gibbon, Old or New World monkeys. This hybridizes to certain fragments in HinfI and HindIII-digested DNA, but little or no repeat sequence homology is seen with EcoRI, KpnI or PstI-digested DNA. The XbaI repeat is composed of 4 tandem repeats of 171, 170, 171, 170 bp. pF5 hybridizes with all centromeric regions but Y. There is 64% hybridization signal at centromeres, 43% total label on D and G group chromosomes, and no hybridization detected at telomeres. |
| References： | Gray KM, et al. Recent amplification of an alpha satellite DNA in humans. Nucleic Acids Res. 13: 521-535, 1985. PubMed: 2987800 |