微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pPV33H |
|---|---|
| 商品货号： | TS203208 |
| Designations： | pPV33H |
| Depositors： | RL Rodriguez |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.2999999523162840 Vector: pPV33H (plasmid) Promoters: Promoter none Construction: pPV33 (ATCC 37073) Marker(s):ampR Construct size (kb): 3.299999952316284 Features: insert detection: tetR marker(s): ampR promoter: none replicon: pMB1 terminator: tet enhancer: none |
| Applications： | promoter-cloning vector |
| Comments： | There are translation stop codons in all three reading frames between the EcoRI and HindIII cloning sites and the initiation codon for the tetracycline resistance gene. This is a promoter cloning plasmid vector in which functional promoters are identified by conferring tetracycline resistance. This was constructed from pPV33 (ATCC 37073) by replacing the EcoRI site with two EcoRI sites flanking a HindIII site. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | West RW Jr., Rodriguez RL. Construction and characterization of E coli promoter-probe plasmid vectors III. pBR322 derivatives with deletions in the tetracycline resistance promoter region. Gene 20: 291-304, 1982. PubMed: 6299894 |
| Shipped： | freeze-dried |