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| 产品名称： | pSVbeta plasmid in E. coli |
|---|---|
| 商品货号： | TS203251 |
| Designations： | pSVbeta plasmid in E. coli |
| Depositors： | GR Macgregor |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.00 Vector: pSVbeta (plasmid) Promoters: Promoter SV40 early Construction: pUC19, pC4AUG beta-gal, pL1 Marker(s):ampR Construct size (kb): 7.0 Features: insert detection: lacZ marker(s): ampR promoter: SV40 early replicon: pMB1 terminator: SV40 late enhancer: SV40 early |
| Applications： | contains easily purifiable cassette(s) for construction reporter construct vector for other uses vector permitting visual detection of activity in colonies |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.0; HindIII--7.0; EcoRI/HindIII--4.3, 2.7; NotI--3.6 (doublet); BamHI--3.6, 3.2, 0.2. Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining or fluorometric assay. Designed to express high levels of beta-galactosidase or an alternative gene product in COS cells. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors. The lacZ gene may be excised by NotI and replaced with alternative sequences. Recommended ampicillin concentration is 200 ug/ml. The order of the major features in this plasmid is: pUC19 - EcoRI - SV40 enhancer - SV40 early promoter - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - HindIII - pUC19. |
| Media： | ATCC® Medium 1945: Terrific broth (ATCC medium 1743) with 200 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | MacGregor GR, Caskey CT. Construction of plasmids that express E. coli beta-galactosidase in mammalian cells. Nucleic Acids Res. 17: 2365, 1989. PubMed: 2495524 Grant R Macgregor, personal communication |
| Shipped： | freeze-dried |