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| 产品名称： | pJIR751 JIR2439 |
|---|---|
| 商品货号： | TS203283 |
| Designations： | pJIR751 JIR2439 |
| Depositors： | JI Rood |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.95
Vector: pJIR751 (plasmid)
Promoters: Promoter lac
Construction: pJIR418
Marker(s): eryR
Construct size (kb): 5.95
Features: insert detection: lacZ marker(s): ermBP other: rep promoter: lac replicon: pIP404 replicon: pMB1 MCS: EcoRI...HindIII |
| Applications： | expression vector shuttle vector vector permitting construction of fusion proteins vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--3.85, 2.1; PvuI--5.95; SmaI--5.95. The vector contains the following restriction sites (approximate kb from the EcoRI site): BglI--0.20, 2.50; NdeI--0.27, 2.09; PvuI--0.17; PvuII--0.14, 5.78; ScaI--4.45; SmaI--4.98. The erythromycin resistance gene (ermBP), expressed in both C. perfringens and E. coli, was derived from C. perfringens and is inactivated by insertions at the ScaI site. The standard reverse sequencing primer is not useful in this vector due to the duplication of the 181 bp region between pUC18 sites PvuII and EcoRI. Shuttle expression vector derived from pJIR418 (ATCC 77387) permitting expression of antibiotic resistance in both hosts. |
| Media： | ATCC® Medium 2305: 1065 plus erythromycin (150 mcg/ml) |
| Growth Conditions： | Temperature: 37°C |
| References： | Bannnam TL, Rood JI. Clostridium perfringens-Escherichia coli shuttle vectors that carry single antibiotic resistance determinants. Plasmid 29: 233-235, 1993. PubMed: 8356117 |
| Shipped： | frozen |
| Shipping Information： | Shipped in E. coli containing the plasmid. |