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| 产品名称： | pJA72 |
|---|---|
| 商品货号： | TS203480 |
| Designations： | pJA72 |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.8969998359680180 Vector: pJA72 (plasmid) Promoters: Promoter lac Construction: pUC, Tn903 Marker(s):TRP1,kanR Construct size (kb): 4.896999835968018 Features: insert detection: lacZ marker(s): kanR, TRP1 promoter: lac replicon: pMB1, ARSH4 centromere: CEN6 |
| Applications： | YC-type (centromeric) shuttle vector shuttle vector vector for other uses vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--2.8, 1.9; XbaI--3.0, 1.7; HindIII--2.7, 2.1; EcoRV--2.8, 1.9. YC-type shuttle vector that can be used with the Tn3-based transposition system developed in Heffrons laboratory. Permits visual detection of recombinants by the lacZalpha complementation system. The order of the major features in this plasmid is: lacZ/MCS - CEN6 - ARS H4 - TRP1 - kanR - pMB1 ori. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Stephen J Elledge, personal communication |
| Related Products： | component of:ATCC 77261 |
| Shipped： | freeze-dried |