Construct size (kb): 5.230000019073486

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, insert detection: lacZ, primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PFN*
Gene name: profilin II
Contains complete coding sequence?: U
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 2.0
Cross references: DNA Seq. Acc.: L10678; EST: EST06533
Nucleotides 13-291 of the insert correspond to
nucleotides 150-428 of L10678 with 88% identity.
DNA Seq. Acc.: L10678; EST: EST06532
Nucleotides 1-187 of the insert correspond to
nucleotides 1693-1507 of L10678 with 98% identity.
DNA Seq. Acc.: T08640; EST: EST06532; Primer: M13-21; THC
id: THC125637
DNA Seq. Acc.: T08641; EST: EST06533; Primer: M13 Reverse;
THC id: THC104389

Insert lengths(kb): 2.0

Tissue: infant brain, 3 months old (from Bento Soares)

Gene product: profilin 2 PFN*

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; HindIII/NotI--3.3, 1.55, 0.38.

Verified to contain EST06533 by single-pass sequencing.