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| 产品名称： | pCarMV-1C |
|---|---|
| 商品货号： | TS203563 |
| Designations： | pCarMV-1C |
| Species： | Carnation mottle virus |
| Depositors： | TJ Morris |
| Vector： | Construct size (kb): 6.550000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.849999904632568 Gene product: genome, 90%+ complete |
| Insert Size (kb)： | 3.850 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Insert contains the following sites separated by (bp)(approx): EcoRI-145- EcoRV-130- NcoI-430- PvuII-315- BglII-70- XhoI-1600- EcoRI-50- KpnI-50- HindIII-200- HincII-840- PstI. Enzymes not cutting insert include BamHI, SmaI, SalI, PstI and ClaI. Restriction digests of the clone give the following sizes (kb): BamHI--6.5; PstI--3.9, 2.7; EcoRI--3.9, 2.7. Isolated from Chenopodium quinoa. This is not a full-length clone and is not infectious. pUC8 dT-tailed at PstI; cut with BamHI; 1st strand synthesis; dC-tailed; cut with EcoRI. pBR322 dG-tailed at PstI; cut with EcoRI; 752 bp fragment = 2nd strand primer. This was hybridized to dC-tailed cDNA, ligated at EcoRI; 2nd strand synthesis. |
| Classification： | Carmovirus |
| References： | Carrington JC, Morris TJ. Complementary DNA cloning and analysis of carnation mottle virus RNA. Virology 139: 22-31, 1984. Guilley H, et al. Nucleotide sequence and genome organization of carnation mottle virus RNA. Nucleic Acids Res. 13: 6663-6677, 1985. PubMed: 3840587 T J Morris, personal communication |