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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS203590 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | BW19851 |
| Application： | conjugation donor host host useful for transposon mutagenesis transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Preceptrol®： | no |
| Genotype： | RP4-2tet::Mu-1kan::Tn7-integrant uidA(deltaMlu1)::pir+ recA1 creC510 hsdR17 endA1 zbf-5 thi |
| Type Strain： | no |
| Comments： | Donor host for conjugative transfer of oriT-containing plasmids that utilize the R6Kgamma origin of replication. Also useful for transposon mutagenesis. Phenotypes verified: Tn7 lac+ recA pir+ RP4tra+. |
| Medium： | ATCC® Medium 1065: LB Agar/Broth, Miller |
| Growth Conditions： | Temperature: 37.0°C |
| Name of Depositor： | BL Wanner |
| Special Collection： | NCRR Contract |
| References： | Metcalf WW, et al. Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6K gamma origin plasmids at different copy numbers. Gene 138: 1-7, 1994. PubMed: 8125283 Barry L Wanner, personal communication |