微信小陈
微信小章
产品简介
购买须知
| 产品名称： | HEL-311 HEL-31 |
|---|---|
| 商品货号： | TS203594 |
| Designations： | HEL-311 HEL-31 |
| GenBank Number： | L06850 |
| Species： | Homo sapiens, human |
| Depositors： | KT Taylor, RH Lipsky |
| Vector： | Construct size (kb): 6.300000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC18 Intact vector size: 2.686 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: HindIII...EcoRI, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: L08752 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CD36 Gene name: CD36 antigen (collagen type I receptor, thrombospondin receptor) Contains complete coding sequence?: Y Chromosome: 7; Localization: 7 q11.2 Tissue: erythroid leukemia (HEL) cell line Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 1.8 Cross references: DNA Seq. Acc.: L06850 Nucleotides 1-1820 of the insert correspond to nucleotides 1-1820 of L06850. Insert lengths(kb): 1.799999952316284 Tissue: erythroid leukemia (HEL) cell line Gene product: CD36 antigen (collagen type I receptor, thrombospondin receptor) CD36 |
| Insert Size (kb)： | 1.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.05, 1.85, 1.25; EcoRI--2.55, 1.85 (doublet); PstI--2.9, 1.1, 0.67, 0.60, 0.32; PvuII--2.25, 1.85, 1.5, 0.8; XbaI--4.0, 1.85, 0.30. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.68; NdeI--1.10; PstI--0.47, 0.75, 1.29; PvuII--0.56; SphI--1.52; XbaI--1.65. The insert is an alternatively spliced product containing exon 2b in the 5 untranslated region. Contains two inserts, tandemly repeated. Oriented with the 5 end closer to the HindIII site of the polylinker. |
| References： | Taylor KT, et al. Characterization of two alternatively spliced 5-untranslated exons of the human CD36 gene in different cell types. Gene 133: 205-212, 1993. PubMed: 7693552 Kirk T Taylor, personal communication |