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| 产品名称： | pGIKS-BioB |
|---|---|
| 商品货号： | TS203651 |
| Designations： | pGIKS-BioB |
| GenBank Number： | J04423 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | M Follettie |
| Applications： | For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). |
| Vector： | Construct size (kb): 4.1 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript II KS+ Intact vector size: 2.961 Type of vector: phagemid Vector end: XhoI Vector end: EcoRI Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR replicon: pMB1, f1 Cross references: Vector end (5): XhoI Vector end (3): EcoRI |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Escherichia coli Gene symbol: bioB Gene name: biotin synthetase Contains complete coding sequence?: Y Type of DNA: genomic Insert 5 end: Modification: XhoI linker Insert 3 end: Modification: EcoRI linker Insert size (kb): 1.1 Cross references: DNA Seq. Acc.: J04423 Nucleotides 1-1041 of the insert correspond to nucleotides 2012-3052 of J04423. Insert lengths(kb): 1.10 Gene product: biotin synthetase bioB Insert end (5): XhoI linker Insert end (3): EcoRI linker |
| Insert Size (kb)： | 1.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). The insert includes nt 2012 - 3052 of Genbank J04423. The insert contains the following restriction sites (approximate kb from the 5 end): RsaI--0.33; DdeI--0.58, 0.82; SspI--1.02. Restriction digests of the clone give the following sizes (kb): EcoRI--4.1; EcoRI/XhoI--3.0, 1.1; XhoI--4.1. |
| Classification： | Enterobacteriaceae, Escherichia |