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| 产品名称： | pGbetaS1.3 pGBS1.3 |
|---|---|
| 商品货号： | TS203714 |
| Designations： | pGbetaS1.3 pGBS1.3 |
| GenBank Number： | M18054 |
| Species： | Homo sapiens, human |
| Applications： | A 284 bp SstI fragment and a 630 bp HinfI/BglI fragment were used to detect polymorphisms with PstI and PvuII, respectively. |
| Vector： | Construct size (kb): 3.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.7400000095367432 Tissue: reticulocyte Gene product: spectrin, beta, erythrocytic (includes sperocytosis, clinical type I) SPTB Alleles: A1, A2, B1, B2 |
| Insert Size (kb)： | 0.740 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Insert contains the following sites separated by (bp) (approx): (SmaI)- 50- HinfI- 10- HinfI- 100- BglI- 80- SstI- 240- PstI- 50- SstI- 290- HinfI- 20- PstI- 15- (SmaI). Restriction digests of the clone give the following sizes (kb): PstI--3.2, 0.43; HindIII--3.7; EcoRI/HindIII--2.8, 0.84; EcoRI/BamHI--2.85, 0.82; EcoRI--3.7. Insert contains human beta-spectrin cDNA corresponding to an internal region of the protein from beta-9 through beta-12. A 284 bp SstI fragment and a 630 bp HinfI/BglI fragment were used to detect polymorphisms with PstI and PvuII, respectively. Detected a 7.8 kb message in RNA blots from two leukemia cell lines (K562 and HEL). Enzyme(s) not detecting polymorphism: several including BamHI and EcoRI. |
| References： | Prchal JT, et al. Isolation and characterization of cDNA clones for human erythrocyte beta-spectrin. Proc. Natl. Acad. Sci. USA 84: 7468-7472, 1987. PubMed: 3478706 |