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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS203743 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | BNN132 |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Shipping Information： | Distributed: freeze-dried |
| Preceptrol®： | no |
| Genotype： | endA1 gyr96 hsdR17 supE44 relA1 delta(lac-proAB) (F traD36 proA+ proB+ lacIq delta(lacZ)M15) lambdaKC(kan-cre) |
| Type Strain： | no |
| Comments： | Phenotype verified: supE44, kanamycin resistance, and lac-. Host for generating plasmid derivatives from recombinants in bacteriophage lambda vectors that contain lox sites flanking the plasmid portion. Example: lambdaYES. JM107 lysogenic for lambdaKC, a bacteriophage lambda derivative containing the cre gene for the site-specific recombinase from bacteriophage P1 that recognizes lox sites. The phage also contains neo from Tn5, conferring kanamycin resistance. |
| Medium： | ATCC® Medium 1065: LB Agar/Broth, Miller |
| Growth Conditions： | Temperature: 37.0°C |
| Name of Depositor： | SJ Elledge |
| References： | Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010 |