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| 产品名称： | pIIIEx313 tRNA |
|---|---|
| 商品货号： | TS203754 |
| Designations： | pIIIEx313 tRNA |
| Depositors： | DR Engelke |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.5999999046325680 Vector: pIIIEx313 tRNA (plasmid) Promoters: Promoter for in vitro transcription T7 Construction: pRS313 Marker(s):ampR,HIS3 Construct size (kb): 6.599999904632568 Features: insert detection: lacZ marker(s): HIS3 marker(s): ampR promoter: SUP4 tRNA intragenic promoter promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: ARSH4 replicon: f1 replicon: pMB1 restriction site: BamHI restriction site: EcoRI restriction site: SalI terminator: RPR1 centromere: CEN6 |
| Applications： | YC-type (centromeric) shuttle vector vector permitting RNA synthesis in vitro vector permitting expression of small structured RNAs vector permitting production of single-stranded DNA vector permitting visual detection of recombinants vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI 6.6; SalI--6.6; EcoRI--6.6. Cloned inserts can be transcribed from the SUP4 tRNA gene intragenic promoter to produce structured pre-tRNA leaders fused to RNA corresponding to inserted sequences. One of a series of shuttle vectors (TS203754 - 87182) designed for stable expression of small structured RNAs in yeast. The vectors differ in promoter/terminator cassettes, selectable markers, replicons, and copy number. Constructed by insertion of a promoter/terminator cassette into the multiple cloning site of the shuttle vector pRS313 (ATCC 77142). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Good PD, Engelke DR. Yeast expression vectors using RNA polymerase III promoters. Gene 151: 209-214, 1994. PubMed: 7828876 |
| Shipped： | freeze-dried |