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| 产品名称： | Influenza A virus (H1N1) |
|---|---|
| 商品货号： | TS203755 |
| Classification： | Orthomyxoviridae, Influenzavirus A |
| Agent： | Influenza A virus (H1N1) |
| Strain： | A/Virginia/ATCC1/2009 |
| Applications： | Emerging infectious disease research Respiratory research Influenza Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Item analyzed using Real-time RT-PCR to determine strain is of pandemic swine Influenza A origin. |
| Effect on Host： | CPE, rounded refractile cells with cell degeneration |
| Recommended Host： | MDCK (ATCC® CCL-34™x99) |
| Growth Conditions： | Recommendations for Infection: xa0Plate cells 24 hours in advance and infect when cultures are 80-90% confluent.xa0xa0 Remove medium and inoculate with a small volume of virus diluted with VGM to provide a MOI 0.1. Adsorb for 1 hour at 35°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: Incubate infected culture for 2 to 5 days at 35°C in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture. |
| Name of Depositor： | ATCC® |
| Special Collection： | ATCC |
| Source： | Nasopharyngeal specimen from a patient positive for Flu A in Virginia, 2009. |