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| 产品名称： | pRS405 |
|---|---|
| 商品货号： | TS203787 |
| Designations： | pRS405 |
| Depositors： | P Hieter |
| Biosafety Level： | 1 |
| Host： | Shipped in: E. coli DH5alpha |
| Vector Information： | Size (kb): 5.507 Vector: pRS405 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pBluescriptII KS+ Marker(s):LEU2,ampR Construct size (kb): 5.507 Features: insert detection: lacZ marker(s): LEU2 marker(s): ampR promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: f1 replicon: pMB1 MCS: KpnI...SacI |
| Applications： | YI-type (integrating) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): ClaI--3.95, 1.55; BglI--3.8, 1.7; PvuII--5.0, 0.45. A YI-type shuttle vector with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 Christianson TW, et al. Multifunctional yeast high-copy-number shuttle vectors. Gene 110: 119-122, 1992. PubMed: 1544568 |
| Related Products： | component of:ATCC 87533 component of:ATCC 87536 |
| Shipped： | frozen |
| Shipping Information： | xa0Freeze dried phagemid in E. coli |