微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pK18mob |
|---|---|
| 商品货号： | TS203798 |
| Designations： | pK18mob |
| Depositors： | A Schaefer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.7599999904632570 Vector: pK18mob (plasmid) Construction: pK18, pSUP102 (RP4 mob) Marker(s):kanR Construct size (kb): 3.759999990463257 Features: insert detection: lacZ marker(s): kanR replicon: oriT replicon: oriV MCS: EcoRI...HindIII |
| Applications： | integrating vector mobilizable vector vector containing primer sites useful for sequencing vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.76; HindIII--3.76; PstI--3.76. After mobilization, the plasmid can be maintained by integration into the host chromosome via homologous recombination. Differs from pK19mob (ATCC 87096) only in the orientation of the polylinker. Cloning vector allowing mobilization into a wide range of Gram- and Gram+ bacteria. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Simon R, et al. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio-Technology 1: 784-791, 1983. Schafer A, et al. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145: 69-73, 1994. PubMed: 8045426 |
| Shipped： | frozen |