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| 产品名称： | lambdaSH3 pcD-GALT |
|---|---|
| 商品货号： | TS203817 |
| Designations： | lambdaSH3 pcD-GALT |
| GenBank Number： | M13430 |
| Species： | Homo sapiens, human |
| Depositors： | M Lefranc, TH Rabbitts |
| Vector： | Construct size (kb): 48.29999923706055 |
| Insert： | DNA: genomic Insert lengths(kb): 18.79999923706055 Tissue: B lymphocyte (EBV transformed), SH cell line Gene product: T cell receptor, gamma, variable region V3 TCRGV1 |
| Insert Size (kb)： | 18.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | Restriction digests of the clone give the following sizes (kb): KpnI--17.5, 10.0 (doublet), 4.9, 3.9, 1.5; PvuI--23.0, 13.0, 8.0; SacI--20.0, 9.0, 6.0, 4.7, 4.4, 1.5, 1.1; XbaI--20.0, 19.0, 9.0; XhoI--20.0, 16.0, 9.0, 2.3. The insert contains the following restriction sites (kb from the 5 end): SacI--0.6, 0.8, 7.0, 8.6, 13.1, 17.7, 18.8 (linker). Contains the genes in the germ line configuration, beginning about 9 kb upstream of pseudogene Vgamma1 and ending just after Vgamma3. The insert extends 5.6 kb further in the 5 direction and is 1.3 kb shorter at the 3 end than the insert in lambdaSH2, and extends 1.3 kb further in the 5 and is 0.2 kb shorter at the 3 end than lambdaSH4. Additional GenBank/EMBL accession numbers: M12949, M13429. |
| References： | LeFranc MP, et al. Diversity and rearrangement of the human T cell rearranging gamma genes: nine germ-line variable genes belonging to two subgroups. Cell 45: 237-246, 1986. PubMed: 2938743 Marie-Paule Lefranc, personal communication |