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| 产品名称： | pSK8 |
|---|---|
| 商品货号： | TS203865 |
| Designations： | pSK8 |
| Species： | Homo sapiens, human |
| Depositors： | M Barbacid, S Katzav |
| Vector： | Construct size (kb): 5.260000228881836 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.299999952316284 Tissue: tumor DNA activated during transfection Gene product: vav 1 oncogene VAV |
| Insert Size (kb)： | 2.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.3; ClaI--4.2, 1.37; BamHI--5.25. The proto-vav gene is not expressed at detectable levels in epithelial, mesenchymal or neuroectodermal cell lines but is readily detected in cell lines of hematopoietic origin. A partial cDNA clone corresponding to nucleotides 593 to 2765 (with ATG initiator codon at nt 111). The probe was generated by the depositors while both were at the Frederick Cancer Research Facility, Frederick, MD 21701. The insert was cloned from a third cycle NIH 3T3 cell transfectant originally transformed with DNA derived from human esophageal carcinoma. The vav oncogene was activated during gene transfer. |
| References： | Katzav S, et al. VAV, a novel human oncogene derived from a locus ubiquitously expressed in hematopoietic cells. EMBO J. 8: 2283-2290, 1989. PubMed: 2477241 Mariano Barbacid, personal communication |