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| 产品名称： | R3 33-10ras3 |
|---|---|
| 商品货号： | TS203954 |
| Organism： | Rattus norvegicus, rat |
| Cell Type： | neuronal Schwann cell; immortalized with SV40 large T antige |
| Product Format： | frozen |
| Morphology： | neuronal |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain SV40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | At the restrictive temperature, expression of large T antigen was not detected PubMed: 3049071. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Tumorigenic： | Yes |
| Effects： | Yes, the cells form colonies in soft agar Ref   Ridley AJ, et al. Ras-mediated cell cycle arrest is altered by nuclear oncogenes to induce Schwann cell transformation. EMBO J. 7: 1635-1645, 1988. PubMed: 3049071 |
| Comments： | AntigenExp: SV40-tsA58 large T antigen PubMed:3049071 Oncogene: v-Ha-rasPubMed:3049071 Products: Myelin-associated glycoprotein (Mag); Protein and mRNA, negative PubMed: 12210843 Myelin basic protein (Mbp); Protein and mRNA, negative PubMed: 12210843 Myelin protein zero (Mpz) (Charcot-Marie-Tooth neuropathy 1B); Protein and mRNA, negative PubMed: 12210843 peripheral myelin protein 22 (Pmp22); protein, negative; mRNA, positive; PubMed: 12210843 S100 calcium-binding protein, beta (neural) (S100b) protein, positive PubMed: 12210843Receptors: Nerve growth factor receptor (Ngfr) Protein and mRNA, negative Comments: Primary cells were infected with the recombinant retrovirus LJ-tsSVLT containing the temperature sensitive tsA58 large SV40 T oncogene and a gene encoding G418 resistance. Clones were selected in the presence of 0.4 mg/ml G418 and super-infected with Zipras 6 containing v-Ha-ras at the permissive temperature of 33C PubMed:3049071. The cells proliferate at both the permissive temperature of 33C and the restrictive temperature of 39.5C. However, the growth rate is increased and growth factor requirements are reduced at the permissive temperature PubMed: 3049071. At the restrictive temperature, expression of large T antigen was not detected PubMed: 3049071. Expression of p21-v-Ha-ras was not detected PubMed: 3049071. A culture submitted to the ATCC in December of 2002 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 33.0°C |
| Temperature Effects： | Restrictive temperature: 39.5°C yes Permissive temperature: 33°C yes |
| Name of Depositor： | PI Patel |
| References： | Hai M, et al. Comparative analysis of Schwann cell lines as model systems for myelin gene transcription studies. J. Neurosci. Res. 69: 497-508, 2002. PubMed: 12210843 Ridley AJ, et al. Ras-mediated cell cycle arrest is altered by nuclear oncogenes to induce Schwann cell transformation. EMBO J. 7: 1635-1645, 1988. PubMed: 3049071 |