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| 产品名称： | pPLEX |
|---|---|
| 商品货号： | TS204012 |
| Designations： | pPLEX |
| Depositors： | G Sczakiel |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.4499998092651370 Vector: pPLEX (plasmid) Promoters: Promoter lambda PL Construction: ptac-rasv, synthetic oligonucleotides Marker(s):ampR Construct size (kb): 4.449999809265137 Features: marker(s): ampR promoter: lambda PL replicon: pMB1 terminator: lambda tRI enhancer: none |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--4.4; PstI--4.4. Plasmid vector for bacterial expression of nonfusion proteins and protein domains. The synthetic DNA used in construction contains translational start and stop signals and unique restriction sites. The galK marker is expressed from lambda PL. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Sczakiel G, et al. An efficient expression vector (pPLEX) for bacterial systhesis of non-fusion proteins and protein domains. Nucleic Acids Res. 15: 1878, 1987. PubMed: 3029730 |
| Shipped： | freeze-dried |