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微信小章| 产品名称： | Koi herpesvirus |
|---|---|
| 商品货号： | TS204258 |
| Classification： | Herpesviridae, unclassified |
| Agent： | Koi herpesvirus |
| Strain： | F347 |
| Common Name： | Koi herpesvirus |
| Applications： | Deposited as a useful research reagent for study of this recently identified pathogen affecting fisheries around the world. |
| Biosafety Level： | 2 BSL 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Isolated by K. Way, CEFAS, in England, 2004 |
| Product Format： | frozen |
| Storage Conditions： | xa0-70°C or colder |
| Comments： | Deposited as a useful research reagent for study of this recently identified pathogen affecting fisheries around the world. This particular isolate has not been sequenced, but several other representatives of this agent have been (Hutoran M, et al. 2005). |
| Effect on Host： | Nephritis, gill necrosis and death in vivo. Intracellular vacuolation in infected cell culture in vitro. |
| Recommended Host： | Recommended Host: xa0CCB cells (cyprinus carp brain) Production Host: Cyprinus carp brain (CCB) cells |
| Growth Conditions： | 7-14 days at 20°C in EMEM supplemented with 10% FBS, L-glutamine and non-essential amino acids |
| Cryopreservation： | |
| Name of Depositor： | C. Hartley |
| Year of Origin： | 2004 |
| References： | Hutoran M., et al. Description of an as yet unclassified DNA virus from diseased cyprinus carpio species. J. Virol. 79: 1983-1991, 2005. PubMed: 15681400 Bercovier, H, et al. Cloning of the koi herpesvirus (KHV) gene encoding thymidine kinase and its use for a highly sensitive PCR based diagnosis. BMC Microbiology 5: 13, 2005. PubMed: 15774009 Waltzek, TB, et al. Koi herpesvirus represents a third cyprinid herpesvirus (CyHV-3) in the family Herpesviridae. J. Gen. Virol. 86: 1659-1667, 2005. PubMed: 15914843 Gilad O., et al. Molecular comparison of isolates of an emerging fish pathogen, koi herpesvirus, and the effect of water temperature on mortality of experimentally infected koi. J. Gen. Virol. 84: 2661-2668, 2003. PubMed: 13679599 |