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| 产品名称： | Nup475 |
|---|---|
| 商品货号： | TS204346 |
| Designations： | Nup475 |
| GenBank Number： | J05669 |
| Species： | Mus musculus, mouse |
| Depositors： | D Nathans |
| Vector： | Construct size (kb): 4.684000015258789 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.814000010490418 Tissue: 3T3 cell line Gene product: Nup475 protein (immediate early nuclear protein)( NUP475, tristetraprolin, zinc finger protein homologous to Zfp-36 in mouse) Nup475 |
| Insert Size (kb)： | 1.814 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.7, 0.7, 0.4; BglI--2.7, 1.8, 0.35; EcoRI--3.0, 1.8; PvuII--4.7; SacI--3.3, 1.4. The insert contains the following restriction sites (kb from the 5 end): BglI--0.199, 0.508; SacI--0.338; BamHI--0.854, 1.195. Detects mouse genomic restriction fragments of the following sizes (kb): EcoRI--8.8; HindIII--7.1. Simple banding patterns were also detected with human and rat DNA. Contains the complete coding sequence and 3 untranslated region and is missing 16 bp from the 5 untranslated region. The ORF of 959 nt begins at nt 60 of the complete mRNA. The insert is oriented such that transcription from the SP6 promoter gives the sense strand. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | DuBois RN, et al. A growth-factor inducible nuclear protein with a novel cysteine/histidine repetitive sequence. J. Biol. Chem. 265: 19185-19191, 1990. PubMed: 1699942 Daniel Nathans, personal communication |